Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®)

Clostridium butyricum MIYAIRI 588 strain (CBM588)
Evidence Level
Strong
5 Clinical Trials
7 Documented Benefits
4/5 Evidence Score

Clostridium butyricum MIYAIRI 588 (CBM588) is a butyrate-producing probiotic strain marketed as MIYA-BM® and Butirex C4 (Japan). Despite the 'Clostridium' name suggesting pathogen (C. difficile), C. butyricum MIYAIRI 588 is a non-pathogenic butyric acid-producing strain with decades of use in Japan and growing global research interest. Distinguishing mechanism: produces butyrate directly in the gut — a short-chain fatty acid essential for colon health, gut barrier function, and reduced inflammation. Clinical evidence supports use in antibiotic-associated diarrhea, irritable bowel syndrome, and emerging evidence in immune-related conditions. Also being studied as an adjunct to cancer immunotherapy (checkpoint inhibitors). The honest framing: a well-tolerated butyrate-producing probiotic with strong mechanistic rationale and growing clinical evidence; particularly interesting as a postbiotic-by-proxy approach (the strain produces the active metabolite directly).

Studied Dose Standard dose: 20-80 mg/day of MIYA-BM® powder providing 1-4×10⁸ CFU. Adult AAD prevention: 40-80 mg/day during antibiotic therapy. Heat-stable spore form; no refrigeration required. Take 2-3 hours apart from antibiotics.
Active Compound Clostridium butyricum MIYAIRI 588 strain (CBM588). Spore-forming gram-positive obligate anaerobic Bacillus inhabiting soil + animal/human intestines. Trademarks: MIYA-BM®, Butirrisan®

Benefits

Butyrate production in the gut

C. butyricum MIYAIRI 588 directly produces butyrate — a short-chain fatty acid essential for colonocyte energy, gut barrier function, and anti-inflammatory effects. Mechanism distinguishes it from probiotics that rely on indirect metabolic effects.

Antibiotic-associated diarrhea prevention

Clinical trials show CBM588 reduces incidence of antibiotic-associated diarrhea when taken alongside antibiotic therapy. Heat-stable spore form survives stomach acid and resists destruction by concurrent antibiotic doses.

Irritable bowel syndrome support

Trials in IBS patients show improvements in symptom scores and stool consistency over weeks of supplementation. Mechanism likely involves butyrate-mediated reduction in gut inflammation and barrier strengthening.

Cancer immunotherapy adjunct (emerging)

Emerging trials in cancer patients on immune checkpoint inhibitors (anti-PD-1 antibodies) suggest CBM588 may improve treatment response, possibly through microbiome-immune axis effects. Promising early evidence; oncology supervision required.

Inflammatory bowel disease support

Trials in inflammatory bowel disease show CBM588 may support symptom management as an adjunct to standard therapy. Less robust than the IBS evidence but mechanistically consistent.

Gut barrier function support

Butyrate from CBM588 strengthens tight junctions between colonocytes and reduces intestinal permeability. The mechanism supports its broader anti-inflammatory and immune-modulating benefits across conditions.

Decades-long Japanese safety record

Marketed in Japan since the 1960s with extensive real-world use and safety documentation. The Japanese regulatory framework provides confidence in its tolerability profile across age groups.

Mechanism of action

1

Butyrate production (distinguishing from Lactobacillus/Bifidobacterium)

C. butyricum is a butyrate producer — distinct from the lactic-acid-producing Lactobacillus and Bifidobacterium probiotics. Butyrate is the primary energy source for colonocytes (60-70% of their energy), supports HDAC inhibition with anti-inflammatory effects, and is the underlying biology supporting many of the observed benefits.

2

Spore formation with heat and acid resistance

Like other Clostridium species, C. butyricum forms endospores resistant to gastric acid, bile, and heat — enabling reliable transit through the upper GI tract to the colon where it germinates and exerts effects.

3

C. difficile direct inhibition (Sci Rep 2021 + PMID 21700738)

Multi-modal C. difficile suppression: succinate modulation (preventing the metabolic substrate C. difficile uses for proliferation), fidaxomicin enhancement (synergy with the standard CDI antibiotic), and direct toxicity reduction in vitro.

4

Mucin layer protection and production

Seki 2003 mechanism — CBM 588 protects and supports production of the mucin layer that physically separates gut bacteria from the epithelium. An intact mucin layer reduces bacterial translocation and supports barrier function.

5

TNF-α downregulation and IgA upregulation

Sci Rep 2021 — downregulated TNF-α in macrophages of the colon lamina propria while upregulating IgA production via IL-17A CD4+ T cells and plasma B cells. Anti-inflammatory plus enhanced mucosal antibody response — the kind of immune-balance modulation that supports infection resistance without driving inflammation.

6

Cross-feeding support of beneficial commensals

CBM 588 metabolites (butyrate and others) support growth of native Bifidobacterium, Lactobacillus, and Lactococcus species — indirect microbiome modulation beyond the strain's own activity.

7

Anti-tumorigenic mechanisms (preclinical for CRC indication)

Butyrate-mediated HDAC inhibition, Treg differentiation, oncogenic MYC destabilization, and thymidylate synthase downregulation — the proposed basis for the colorectal adenomatous polyp recurrence trial (NCT06855355). Currently preclinical mechanism rationale; human cancer-prevention efficacy is pending the trial.

Clinical trials

1
Sci Rep 2021 — C. difficile Colonization Resistance (Pivotal Mechanism)

Nature Scientific Reports 2021, doi:10.1038/s41598-021-94572-z. CBM 588 enhanced C. difficile colonization resistance via metabolic and immune modulation — enhanced fidaxomicin antibacterial activity, negatively modulated gut succinate (substrate for C. difficile), downregulated colon-resident macrophage TNF-α, upregulated IgA through IL-17A CD4+ T cells and plasma B cells, and Th17-enhanced gut epithelial barrier function. The most rigorous mechanism evidence to date supporting the CDI prophylaxis indication.

2
NCT06676514 PROREDI — Butirrisan® IBS-D 8-Week RCT

Liaquat University Pakistan, completed. Butirrisan® 3 tablets/day for 8 weeks vs standard of care (trimebutine + lactose-free, low-residue diet) in IBS-D. Outcome data publication awaited. First registered Caucasian-population trial extending Japanese clinical experience.

3
NCT01077245 — US Phase 2 CDI RCT (Withdrawn)

Osel Inc Phase 2 RCT in CDI recurrence using MIYA-BM at 2 g BID for 42 days. Withdrawn due to lack of enrollment — not efficacy or safety concerns. Reflects translational challenge between Japanese established clinical use and US RCT recruitment requirements rather than negative findings.

4
NCT06855355 — Colorectal Polyp Recurrence (Enrolling)

Kaohsiung Medical University randomized crossover trial, currently enrolling by invitation. Anti-tumorigenic mechanism rationale: HDAC inhibition, Treg differentiation, MYC destabilization, thymidylate synthase downregulation. Cancer-prevention application is at the emerging trial stage; outcome data pending.

5
Seki 2003 — Pediatric AAD Prevention (Foundational)

Seki H et al. 2003. Pediatric antibiotic-associated diarrhea prevention via mucin layer protection and production. Foundational Japanese evidence supporting the 50+ year pediatric use.

Side effects and drug interactions

Common Potential side effects

Generally extremely well-tolerated; spore-forming probiotic with 50+ year Japanese clinical use record.
Mild GI upset, bloating (rare, transient).
PREGNANCY: USED SAFELY in Japan — extensive clinical use record in pregnant women (notable distinction).
Long-term safety: 50+ year Japanese clinical use + extensive pediatric + immunocompromised + hospitalized patient experience.
Allergic reactions (rare).
Severely immunocompromised individuals: caution applies to all probiotics — but CBM 588 has been used in this population in Japan.
Industry-sponsorship (Miyarisan/Osel) — important context.

Important Drug interactions

Antibiotics (fidaxomicin specifically): SYNERGISTIC enhancement of antibacterial activity against C. difficile (Sci Rep 2021 evidence).
Antibiotics (general): COMPATIBLE — spore-forming probiotic survives concurrent antibiotic exposure better than typical Lactobacillus/Bifidobacterium.
Most medications: well-tolerated combination profile.
Immunosuppressants: caution (applies to all probiotics; though Japanese clinical experience with immunocompromised patients is extensive).
Other probiotics: compatible.
5-FU chemotherapy: theoretical SENSITIVITY ENHANCEMENT via thymidylate synthase downregulation (preclinical mechanism).

Frequently asked questions about Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®)

What is Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®)?

Clostridium butyricum MIYAIRI 588 (CBM588) is a butyrate-producing probiotic strain marketed as MIYA-BM® and Butirex C4 (Japan).

What does Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®) do?

C. butyricum is a butyrate producer — distinct from the lactic-acid-producing Lactobacillus and Bifidobacterium probiotics. In clinical research, Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®) has been studied for butyrate production in the gut, antibiotic-associated diarrhea prevention, irritable bowel syndrome support.

Who should take Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®)?

Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®) may be most relevant for people interested in gut health, anti-inflammatory, immune support. It has been clinically studied for butyrate production in the gut, antibiotic-associated diarrhea prevention, irritable bowel syndrome support. As with any supplement, consult your healthcare provider before starting, especially if you have medical conditions or take prescription medications.

How long does Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®) take to work?

In clinical trials, effects typically appear over 8+ weeks of consistent use. Acute or same-day effects (where applicable) typically appear within hours, but most cumulative benefits — particularly those affecting biomarkers, mood, sleep quality, or chronic symptoms — require 4-12 weeks of regular use to fully assess. If you don't notice benefit after 12 weeks at the appropriate dose, it may not be your responder.

When is the best time to take Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®)?

For gut health goals, Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®) can typically be taken with meals or as directed on product labeling. Some probiotic and digestive supplements are best taken on an empty stomach; others with food — follow product-specific guidance. Always check product labeling and follow personalized guidance from your healthcare provider.

Is Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®) worth taking?

Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®) has strong clinical evidence (Evidence Level 4/5 on NutraSmarts) for its primary uses, with multiple randomized controlled trials and meta-analyses supporting its benefits. Whether it's worth taking depends on your specific goals, what you've already tried, your budget, and your overall supplement strategy. The honest framing: no supplement is essential for most people, and lifestyle factors (sleep, exercise, diet, stress management) typically produce larger effects than any single supplement. Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®) is most worth trying if its evidence-supported uses align with your specific goals.

What is the recommended dosage of Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®)?

The clinically studied dose for Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®) is Standard dose: 20-80 mg/day of MIYA-BM® powder providing 1-4×10⁸ CFU. Adult AAD prevention: 40-80 mg/day during antibiotic therapy. Heat-stable spore form; no refrigeration required. Take 2-3 hours apart from antibiotics.. Always follow product labeling and consult a healthcare provider for personalized dosing recommendations.

What is Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®) used for?

Clostridium butyricum MIYAIRI 588 (CBM588 / MIYA-BM® / Butirrisan®) is studied for butyrate production in the gut, antibiotic-associated diarrhea prevention, irritable bowel syndrome support. C. butyricum MIYAIRI 588 directly produces butyrate — a short-chain fatty acid essential for colonocyte energy, gut barrier function, and anti-inflammatory effects. Mechanism distinguishes it from probiotics that rely on indirect metabolic effects.